After selecting 47 farms with colonized pigs in different regions of Germany we sampled the nares of 113 humans working daily with pigs and of their 116 non exposed family members.
The same was performed in 18 veterinarians attending pig farms and in 44 of their non exposed family members.
For investigating transmission beyond families we samples the nares of 462 pupils attending a secondary school in a high density pig farming area. MRSA were detected by direct culture on selective agar.
The isolates were typed by means of spa-sequence typing and classification of SCCmec elements. For attribution of spa sequence types to clonal lineages as defined by multi locus sequence typing we used the BURP algorithm. Antibiotic susceptibility testing was performed by microbroth dilution assay.
Christiane Cuny1, Rolf Nathaus3, Franziska Layer1, Birgit Strommenger1, Doris Altmann2, Wolfgang Witte